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Isolation and molecular characterization of Fusarium species (Fungi, Ascomycota) from unhatched eggs of Caretta caretta in Tuscany (Italy)

  • Samuele Risoli
  • Sabrina Sarrocco
  • Giuliana Terracciano
  • Riccardo Baroncelli
  • Marco Alberto Luca Zuffi
  • Cecilia Mancusi
  • Cristina Nali

Caretta caretta is the only sea turtle species nesting along the Tuscan coastline, where nests are becoming more numerous and widespread, as well as the number of Sea Turtle Egg Fusariosis (STEF)-affected eggs. In this work, we analyzed eggs from natural nests of C. caretta that showed visual symptoms of STEF from Tuscan nests, with the aim to (i) isolate Fusarium spp. strains present in unhatched eggs, (ii) morphologically and molecularly characterize the isolated fungi and, finally, (iii) reconstruct the phylogenetic relationships between our isolates and those already known.

  • Keywords:
  • Emerging infectious diseases,
  • Sea Turtle Egg Fusariosis,
  • Fusarium Solani Species Complex,
  • Fusarium,
  • Caretta caretta,
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Samuele Risoli

University of Pisa, Italy - ORCID: 0000-0002-6232-3775

Sabrina Sarrocco

University of Pisa, Italy - ORCID: 0000-0002-7080-8369

Giuliana Terracciano

Istituto Zooprofilattico Sperimentale, Italy

Riccardo Baroncelli

University of Bologna, Italy - ORCID: 0000-0002-5878-1159

Marco Alberto Luca Zuffi

University of Pisa, Italy - ORCID: 0000-0003-1710-9929

Cecilia Mancusi

ARPAT, Italy - ORCID: 0000-0002-7322-1985

Cristina Nali

University of Pisa, Italy - ORCID: 0000-0002-8228-0258

  1. Summerell, B. A. (2019). Resolving Fusarium: current status of the genus. Annu. Rev. Phytopathol. 57, 323 - 339 DOI: 10.1146/annurev-phyto-082718-100204
  2. Dean R., van Kan J. A. L., Pretorius Z. A., Hammond-Kosack K. E., di Pietro A., Spanu P. D., Rudd J. J., Dickman M., Kahmann R., Ellis J., Foster, G. D. (2012) - The Top 10 fungal pathogens in molecular plant pathology. Mol. Plant Pathol. 13, 414 - 430 DOI: 10.1111/j.1364-3703.2011.00783.x
  3. D’Mello, J. P. F., Placinta C. M., Macdonald A. M. C. (1999) - Fusarium mycotoxins: a review of global implications for animal health, welfare and productivity. Anim. Feed Sci. Technol. 80, 183 - 205 DOI: 10.1016/S0377-8401(99)00059-0
  4. Sarrocco S., Mauro A., Battilani P. (2019): Use of Competitive Filamentous Fungi as an Alternative Approach for Mycotoxin Risk Reduction in Staple Cereals: State of Art and Future Perspectives. Toxins 11, 701 - 701 DOI: 10.3390/toxins11120701
  5. Zhang N., O’Donnell K., Sutton D. A., Nalim F. A., Summerbell R. C., Padhye A. A., and Geiser D. M., (2006) - Members of the Fusarium solani species complex that cause infections in both humans and plants are common in the environment. J. Clin. Microbiol. 44, 2185 - 2190 DOI: 10.1128/jcm.00120-06
  6. Short D. P. G., O’Donnell K., Zhang N., Juba J. H., Geiser D. M. (2011): Widespread occurrence of diverse human pathogenic types of the fungus Fusarium detected in plumbing drains. J. Clin. Microbiol. 49, 4264 - 4272 DOI: 10.1128/2FJCM.05468-11
  7. Fisher M. C., Henk D. A., Briggs C. J., Brownstein J. S., Madoff L. C., McCraw S. L., Gurr S. J., (2012) - Emerging fungal threats to animal, plant and ecosystem health. Nature 484, 186 - 194 DOI: 10.1038/nature10947
  8. Sandoval-Denis M., Crous P. W. (2018). Removing chaos from confusion: assigning names to common human and animal pathogens in Neocosmospora. Persoonia 41, 109 - 129 DOI: 10.3767/2Fpersoonia.2018.41.06
  9. Groner M. L., Maynard J., Breyta R., Carnegie R. B., Dobson A., Friedman C. S., Froelich B., Garren M., Gulland F. M. D., Heron S. F., Noble R. T., Revie C. W., Shields J. D., Vanderstichel R., Weil E., Wyllie-Echeverria S., Harvell C. D. (2016) - Managing marine disease emergencies in an era of rapid change. Philos. Trans. R. Soc. B: Biol. Sci. 371 DOI: 10.1098/rstb.2015.0364
  10. Casale, P. & Tucker, A.D. (2017). Caretta caretta (amended version of 2015 assessment). The IUCN Red List of Threatened Species 2017
  11. Smyth, C.W., J.M. Sarmiento-Ramírez, D.P.G. Short, J. Diéguez-Uribeondoid, K. O’donnell, and D.M. Geiser, 2019: Unraveling the ecology and epidemiology of an emerging fungal disease, sea turtle egg fusariosis (STEF). PLoS Pathogens 15 DOI: 10.1371/journal.ppat.1007682
  12. Gleason F. H., Allerstorfer M., Lilje O. (2020) - Newly emerging diseases of marine turtles, especially sea turtle egg fusariosis (SEFT), caused by species in the Fusarium solani species complex (FSSC). Mycology 11, 184 - 194 DOI: 10.1080/2F21501203.2019.1710303
  13. Sarmiento-Ramírez J. M., Abella E., Martín M. P., Tellería M. T., López-Jurado L. F., Marco A., Diéguez-Uribeondo J. (2010) - Fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, Caretta caretta, in Boavista, Cape Verde. FEMS Microbiol. Lett. 312, 192 – 200 DOI: 10.1111/j.1574-6968.2010.02116.x
  14. Sarmiento-Ramírez, J. M., Abella-Pérez E., Phillott A. D., Sim J., van West P., Martín M. P., Marco A., Diéguez-Uribeondo J. (2014): Global distribution of two fungal pathogens threatening endangered sea turtles. PLoS ONE 9 DOI: 10.1371/journal.pone.0085853
  15. Phillott A. D., Parmenter C. J., McKillup S. C. (2006) - Calcium depletion of eggshell after fungal invasion of sea turtle eggs. Chelonian Conserv. Biol. 5, 146 - 149 DOI: 10.2744/1071-8443(2006)5[146:CDOEAF]2.0.CO;2
  16. Baroncelli R., Sarrocco S., Zapparata A., Tavarini S., Angelini L. G., Vannacci G. (2015) - Characterization and epidemiology of Colletotrichum acutatum sensu lato (C. chrysanthemi) causing Carthamus tinctorius anthracnose. Plant Pathol. 64, 375 - 384 DOI: 10.1111/ppa.12268
  17. Cobo-Díaz J. F., Baroncelli R., Le Floch G., Picot A. (2019). A novel metabarcoding approach to investigate Fusarium species composition in soil and plant samples. FEMS Microbiol. Ecol. 95 DOI: 10.1093/femsec/fiz084
  18. Katoh K., Misawa K., Kuma K. I., Miyata T. (2002). MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res., 30, 3059- 3066 DOI: 10.1093/nar/gkf436
  19. Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35, 1547 DOI: 10.1093/molbev/msy096
  20. Huelsenbeck, J. P., & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754 - 755 DOI: 10.1093/bioinformatics/17.8.754
  21. Candan A. Y., Katılmış Y., Ergin Ç. (2021). First report of Fusarium species occurrence in loggerhead sea turtle (Caretta caretta) nests and hatchling success in Iztuzu Beach, Turkey. Biologia, 76(2), 565-573 DOI: 10.2478/s11756-020-00553-4
  22. Felici L., Francesconi S., Balestra G. G., (2021). First report of Fusarium nodosum (L. Lombard & Crous) on durum wheat (Triticum turgidum subsp. durum) in Italy. Proceedings of the 26° SIPaV Congress, Virtual Congress, September 15-17, 2021
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  • Publication Year: 2022
  • Pages: 747-755

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  • Publication Year: 2022

Chapter Information

Chapter Title

Isolation and molecular characterization of Fusarium species (Fungi, Ascomycota) from unhatched eggs of Caretta caretta in Tuscany (Italy)

Authors

Samuele Risoli, Sabrina Sarrocco, Giuliana Terracciano, Riccardo Baroncelli, Marco Alberto Luca Zuffi, Cecilia Mancusi, Cristina Nali

Language

English

DOI

10.36253/979-12-215-0030-1.71

Peer Reviewed

Publication Year

2022

Copyright Information

© 2022 Author(s)

Content License

CC BY-NC-SA 4.0

Metadata License

CC0 1.0

Bibliographic Information

Book Title

Ninth International Symposium “Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques”

Book Subtitle

Livorno (Italy) 14th-16th June 2022

Editors

Laura Bonora, Donatella Carboni, Matteo De Vincenzi, Giorgio Matteucci

Peer Reviewed

Publication Year

2022

Copyright Information

© 2022 Author(s)

Content License

CC BY-NC-SA 4.0

Metadata License

CC0 1.0

Publisher Name

Firenze University Press

DOI

10.36253/979-12-215-0030-1

eISBN (pdf)

979-12-215-0030-1

eISBN (xml)

979-12-215-0031-8

Series Title

Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques

Series ISSN

2975-0423

Series E-ISSN

2975-0288

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