Material and Methods
- Liliana Napolitano
- © 2026 Author(s) |
- CC BY 4.0
- DOI: 10.36253/979-12-215-0993-9.03
This chapter describes the materials and methods employed across the three main sections of the study. It details patient recruitment and neuropsychological examination, CSF collection, and biochemical assays such as circular dichroism (CD), dynamic light scattering (DLS), and intrinsic tryptophan fluorescence. The methodological framework also encompasses cell culture models, intracellular calcium measurements, MTT reduction assays, and advanced imaging techniques like STED microscopy. Furthermore, it outlines the purification of DesAb-O and Dimeric-DesAb-O constructs, including the characterization of their binding properties through dot blot analysis and Real-Time based ELISA assay. Furthermore, statistical methodologies and ROC curve analyses used for data evaluation are detailed.
- Keywords:
- Experimental Methods,
- CSF Analysis,
- STED Microscopy,
- Cell Culture,
- Biochemical Assays,
- Statistical Analysis,
- ORCID: 0009-0004-3087-286X
Chapter Information
Chapter Title
Material and Methods
Authors
Liliana Napolitano
Language
English
DOI
10.36253/979-12-215-0993-9.03
Peer Reviewed
Publication Year
2026
Copyright Information
© 2026 Author(s)
Content License
Metadata License
Bibliographic Information
Book Title
A multidisciplinary approach for the early diagnosis of Alzheimer’s disease and potential therapeutic applications
Authors
Liliana Napolitano
Peer Reviewed
Number of Pages
184
Publication Year
2026
Copyright Information
© 2026 Author(s)
Content License
Metadata License
Publisher Name
Firenze University Press
DOI
10.36253/979-12-215-0993-9
ISBN Print
979-12-215-0992-2
eISBN (pdf)
979-12-215-0993-9
eISBN (xml)
979-12-215-0994-6
Series Title
Premio Tesi di Dottorato Città di Firenze
Series ISSN
3103-3881
Series E-ISSN
3103-3989