Second Section: A single domain antibody detects and neutralises toxic Aβ42 oligomers in the Alzheimer’s disease CSF
- Liliana Napolitano
- © 2026 Author(s) |
- CC BY 4.0
- DOI: 10.36253/979-12-215-0993-9.05
This section focuses on the application of the single-domain antibody DesAb-O as a tool for detecting and neutralizing toxic Aβ42 oligomers. Through in vitro ELISA and dot-blot assays, the study demonstrates that DesAb-O selectively detects synthetic Aβ42 oligomers and recognizes them within the complex matrix of cerebrospinal fluid (CSF) from Alzheimer’s Disease patients. Utilizing STED microscopy, the study further characterizes the antibody’s ability to identify oligomers bound to neuronal membranes and internalized into the cytosol. Crucially, the chapter provides evidence that DesAb-O inhibits the interaction between toxic aggregates and the SH-SY5Y cells, thereby preventing mitochondrial dysfunction, membrane destabilization, and neuronal impairment induced by both synthetic species and patient-derived CSF.
- Keywords:
- Alzheimer’s Disease,
- Aβ42 Oligomers,
- Single-domain Antibody,
- ELISA Assay,
- STED Microscopy,
- Neuronal Dysfunction,
- ORCID: 0009-0004-3087-286X
Chapter Information
Chapter Title
Second Section: A single domain antibody detects and neutralises toxic Aβ42 oligomers in the Alzheimer’s disease CSF
Authors
Liliana Napolitano
Language
English
DOI
10.36253/979-12-215-0993-9.05
Peer Reviewed
Publication Year
2026
Copyright Information
© 2026 Author(s)
Content License
Metadata License
Bibliographic Information
Book Title
A multidisciplinary approach for the early diagnosis of Alzheimer’s disease and potential therapeutic applications
Authors
Liliana Napolitano
Peer Reviewed
Number of Pages
184
Publication Year
2026
Copyright Information
© 2026 Author(s)
Content License
Metadata License
Publisher Name
Firenze University Press
DOI
10.36253/979-12-215-0993-9
ISBN Print
979-12-215-0992-2
eISBN (pdf)
979-12-215-0993-9
eISBN (xml)
979-12-215-0994-6
Series Title
Premio Tesi di Dottorato Città di Firenze
Series ISSN
3103-3881
Series E-ISSN
3103-3989