Development of optical methods for real-time whole-brain functional imaging of zebrafish neuronal activity

  • Lapo Turrini,

Each one of us in his life has, at least once, smelled the scent of roses, read one canto of Dante’s Commedia or listened to the sound of the sea from a shell. All of this is possible thanks to the astonishing capabilities of an organ, such as the brain, that allows us to collect and organize perceptions coming from sensory organs and to produce behavioural responses accordingly. Studying an operating brain in a non-invasive way is extremely difficult in mammals, and particularly in humans. In the last decade, a small teleost fish, zebrafish (Danio rerio), has been making its way into the field of neurosciences. The brain of a larval zebrafish is made up of 'only' 100000 neurons and it’s completely transparent, making it possible to optically access it. Here, taking advantage of the best of currently available technology, we devised optical solutions to investigate the dynamics of neuronal activity throughout the entire brain of zebrafish larvae.

  • Keywords:
  • zebrafish,
  • calcium imaging,
  • light-sheet microscopy,
  • epilepsy,
  • behaviour,
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Lapo Turrini

University of Florence, Italy - ORCID: 0000-0003-2519-7893

Lapo Turrini graduated in 2015 with a Master’s degree in Biology at the University of Florence and received his PhD with highest grades in 2019 for his work in the Biophotonics group at the European Laboratory for Non-Linear Spectroscopy. His main research interests are in the field of neuroscience. In particular, he makes use of cutting-edge custom-made fluorescence microscopes to investigate the neuronal activity of zebrafish larvae during virtual-reality-evoked motor behaviour.
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