Monograph

Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology

  • Roberta Gualdani,

Ion channels are membrane proteins that selectively allow ions to flow down their electrochemical gradient across the cellular membrane. They localize in both plasma and intracellular membranes and regulate a variety of functions such as neuronal excitability, heartbeat, muscle contraction and hormones release. Thus, understanding the molecular mechanism of ion channels function and regulation is one of the key goals of modern Biophysics. During my PhD thesis, by combining patch-clamp measurements with site-direct mutagenesis, fluorophore labeling experiments and pharmacological assays, I explored some functional and structural properties of different ion transporters: the Na+/Ca2+ exchanger (NCX); the large conductance Ca2+-voltage activated K+ channel (BK) channel; the human Transient receptor potential, member A1 (TRPA1) channel.

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Roberta Gualdani

University of Florence, Italy - ORCID: 0000-0003-3617-4953

She graduated in Chemistry at Scuola Normale Superiore in 2008, with a thesis carried out at NEST laboratory in Pisa. She subsequently joined the research team of Prof. Maria Rosa Moncelli (Chemistry Department, University of Florence) in 2009 as PhD student and, since 2012, as postdoctoral fellow. Her project is aiming to investigate how ion channels work and how novel drugs modulate them, by combining fluorescence and electrophysiological techniques.
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  • Publication Year: 2013
  • Pages: 64
  • eISBN: 978-88-6655-453-0
  • Content License: CC BY-ND 3.0 IT
  • © 2013 Author(s)

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  • Publication Year: 2013
  • eISBN: 978-88-9273-470-8
  • Content License: CC BY-ND 3.0 IT
  • © 2013 Author(s)

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  • Publication Year: 2013
  • Pages: 64
  • eISBN: 978-88-6655-452-3
  • Content License: CC BY-ND 3.0 IT
  • © 2013 Author(s)

Bibliographic Information

Book Title

Using the Patch-Clamp technique to shed light on ion channels structure, function and pharmacology

Authors

Roberta Gualdani

Peer Reviewed

Number of Pages

64

Publication Year

2013

Copyright Information

© 2013 Author(s)

Content License

CC BY-ND 3.0 IT

Metadata License

CC0 1.0

Publisher Name

Firenze University Press

DOI

10.36253/978-88-6655-453-0

ISBN Print

978-88-6655-452-3

eISBN (pdf)

978-88-6655-453-0

eISBN (xml)

978-88-9273-470-8

Series Title

Premio Tesi di Dottorato

Series Issn ISSN

2612-8039

Series E-Issn

2612-8020

377

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